Co-Enzymes with Dissimilar Stabilities: A Discussion of the Likely Biocatalyst Performance Problems and Some Potential Solutions
نویسندگان
چکیده
Enzymes have several excellent catalytic features, and the last few years seen a revolution in biocatalysis, which has grown from using one enzyme to multiple enzymes cascade reactions, where product of reaction is substrate for subsequent one. However, stability remains an issue despite many benefits system. When are exposed harsh process conditions, deactivation occurs, changes activity enzyme, leading increase time achieve given conversion. Immobilization well-known strategy improve properties, if immobilization properly designed controlled. Enzyme co-immobilization further step complexity preparing biocatalyst, whereby two or more immobilized on same particle support. One crucial problem when designing co-immobilized possibility with very different stabilities. This paper discusses scenarios differing stability. The effect operational performance shown via simple simulations Michaelis–Menten equations describe kinetics integrated term. Finally, some strategies overcoming these problems discussed.
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ژورنال
عنوان ژورنال: Catalysts
سال: 2022
ISSN: ['2073-4344']
DOI: https://doi.org/10.3390/catal12121570